收藏本站 全国免费服务热线:400-6296615
所有类目 诊断试剂网生殖催乳素

兔子催乳素(PRL)ELISA 试剂盒 国产ELISA 进

1000.00元/件

  • 起订量:0
  • 可售数量:0

面向地区:北京市 北京市

发货期:1天

齐一生物科技(上海)有限公司
薄顺宇先生
手机:
联系我时务必告知是在医健网上看到的!

齐一生物科技(上海)有限公司

商铺| 企业名片
  • 地址:北京市北京市
  • 电话:86-021-60348467

热卖推荐

详情描述

兔子催乳素(PRL)ELISA 试剂盒厂家现货直销,齐一生物科技专业生产研发经营稳定、优质、高效、实用的ELISA试剂盒,公司提供ELISA试剂盒免费代测,ELISA试剂盒批发价零售,ELISA试剂盒专业生产厂家,ELISA试剂盒进口品牌!

最新报价,检测原理,使用说明书,欢迎来电咨询免费索要!
兔子催乳素(PRL)ELISA 试剂盒

英文名称:

Rabbit prolactin,PRL ELISA Kit

产品规格:96T/48T

品牌:进口/国产
兔子催乳素(PRL)ELISA 试剂盒
使用范围:仅供科研检测,不得用于临床。
保存条件:2-8℃
发货方式:专门快递免费送货上门
产品价格:价格优惠,欢迎来电洽谈!
说明书:说明书随货发送,您也可以直接联系我司在线销售人员索取。

特点:灵敏性高、特异性强、重复性好

服务:齐一生物所有的elisa试剂盒均免费代测,全程Elisa实验技术指导,免费代做实验。

咨询:021-60348467  13818942376  QQ:917158874 胡经理

兔子催乳素(PRL)ELISA 试剂盒

Drug Names
Generic Name:

Rabbit prolactin,PRL ELISA Kit


Materials provided with the kit
Materials provided with the kit 48erminations 96 erminations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard:180ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution (20ml×20 fold)
×1bottle (20ml×30 fold)
×1bottle 2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-ect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,ect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t ect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 120ng/L,80ng/L ,40ng/L,20ng/L,10ng/L)
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result ermination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage and validity
1.Storage: 2-8℃.
2.validity: six months.

兔子催乳素(PRL)ELISA 试剂盒

其他相关产品容如下:

无水氯化铜/无水氯化铜(Ⅱ)/Copper(II) chloride AR,98% 250克
1公斤
超纯,99.995% 5克
25克
氯化金/四水合氯金酸/四水合四氯络金氢酸/四氯金酸/氯金水合物/Chloroauric acid hydrated AR,47.8% 1克
无水氯化金/三氯化金/氯金酸/Gold(III) chloride AR,47.8% 1克
5克
六水三氯化铁/氯化高铁/氯化铁/Iron(III)chloride hexahydrate AR,99% 500克
无水三氯化铁/无水氯化高铁/无水氯化铁/Iron(III) chloride CP,97% 500克
氯化镍/六水合氯化镍/氯化亚镍/六水合二氯化镍/镍铬合金氯化物/氯化镍(II)六水合物/Nickel chloride hexahydrate AR,98% 500克
氯化亚锡/二氯化锡/结晶氯化亚锡/氯化锡(II)二水合物/Stannic chloride dihydrate AR,98% 500克
SP,98.5% 5克
无水二氯化锡/无水氯化亚锡/氯化锡(II)/Tin(II) chloride anhydrous AR,98% 100克
四氯化锡/结晶四氯化锡/氯化高锡/五水四氯化锡/结晶氯化锡/Stannic chloride pentahydrate AR,99% 500克
氯化锌/锌氯粉/Zinc chloride AR,98% 500克
氯化铋/三氯化铋/氯化铋(III)/Bismuth Chloride AR,98% 100克
氯化铬/三氯化铬/六水氯化铬/六水合三氯化铬(III)/Chromic chloride hexahydrate AR,99% 500克
无水氯化铬(III)/无水三氯化铬/无水三氯化铬(III)/Chromium(III) chloride anhydrous AR,99% 500克
氯化铟/三氯化铟/Indium chloride AR,39% 10克
4N,99.99% 5克
25克
100克
氯化亚铁/二氯化铁/氯化铁四水合物/四水氯化低铁/氯化铁(II)四水合物/Iron(II) chloride tetrahydrate AR,99% 500克
氯化铅/二氯化铅/氯化铅(Ⅱ)/Lead chloride AR,99.5% 500克
四水/氯化亚锰/二/(II)四水合物/Manganese chloride AR,99% 500克
氯化亚/一氯化/甘/氯化(I)/一氯化亚(I)/氯化亚(I)/Mercuric chloride CP,99% 500克
氯化银/Silver chloride AR,99.5% 25克
100克
氯化锶/六水氯化锶/二氯化锶/Strontium chloride hexahydrate GR,99.8% 500克
AR,99.5% 500克
氯化铷/Rubidium chloride AR,99% 5克
25克
SP,99.5% 1克
无水三氯化钌/氯化钌/三氯化钌/氯化钌(III)/无水氯化物钌(III)/Ruthenium(III) chloride GR,99.5% 1克
水合三氯化钌/氯化钌水合物/三氯化钌水合物/氯化钌(III)水合物/Ruodium (Ⅲ) Chloride Hydrate GR,99.99% 1克
氯化铈/三氯化铈/七水氯化铈(III)/氯化亚铈/硒氯化水合物/Cerium(III) chloride heptahydrate AR,99% 100克
无水氯化铈/三氯化铈/氯化亚铈/氯化铈(Ⅲ)/Cerous chloride AR,99.5% 10克
50克
氯化镧/氯化镧七水合物/三氯化镧/氯化镧(III)七水合物/Lanthanum chloride heptahydrate AR,45% 100克
氯化钕/三氯化钕/氯化钕(III)/Neodymium(III) chloride 2.5N,99.5% 10克
羟高铁血红素/高铁血红素/羟基血红素/血红素/原血红素/苏木红/铁(Ⅲ)原血红素/Hematin 高纯,98% 1克
5克
25克
200/聚氧乙烯200/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯200/PEG200 CP 500克
色固 100克
300/聚氧乙烯300/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯300/PEG300 CP 500克
色固 100克
400/聚氧乙烯400/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯400/PEG400 CP 500克
色固 100克
600/聚氧乙烯600/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯600/PEG600 CP 500克
色固 100克
800/聚氧乙烯800/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯800/PEG800 CP 500克
1000/聚氧乙烯1000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯1000/PEG1000 CP 500克
色固 100克
1500/聚氧乙烯1500/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯1500/PEG1500 AR 500克
色固 100克
2000/聚氧乙烯2000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯2000/PEG2000 CP 500克
色固 100克
4000/聚氧乙烯4000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯4000/PEG4000 CP 500克
色固 100克
6000/聚氧乙烯6000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯6000/PEG6000 CP 500克
色固 100克
8000/聚氧乙烯8000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯8000/PEG8000 CP 500克
10000/聚氧乙烯10000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯10000/PEG10000 AR 250克
20公斤
色固 100克
20000/聚氧乙烯20000/20M/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯20000/PEG20000 AR 250克
15公斤
色固 100克
35000/聚氧乙烯35000/α-氢-ω-羟基(氧-1,2-乙二基)的聚合物/聚氧化乙烯35000/PEG35000 AR 1公斤
/甘醇/亚乙基二醇/1,2-/1,2-亚乙基二醇/乙撑二醇/Ethylene glycol AR,99% 500毫升
色标 2毫升
β-/2-/硫醇基乙醇//1-/2-羟基乙硫醇/β-硫醇代乙醇/一/β-Mercaptoethanol BR,99% 100毫升
1升
巯基乙酸/酸/硫醇代乙酸/氢硫基乙酸/硫氢基乙酸/乙硫醇酸/硫醇醋酸/巯基醋酸/TGA AR,90% 100毫升
聚乙烯醇/PVA 1750±50类型 500克
AH-26类型 250克
粘度11-14cp 250克
聚乙烯醇缩丁醛/聚乙烯醇缩丁醛树脂/聚酸甲基酯/PVB 航空级 500克
1,3-丙二醇/1,3--二羟基丙烷/1,3-Propanediol AR,98% 100毫升

其他催乳素相关产品

更多更多催乳素相关产品

催乳素相关分类:

医建网郑重提醒:过低的价格和夸张的描述有可能是虚假信息,请买家谨慎对待,谨防欺诈。欺诈行为举报邮箱:yjo2o@yjo2o.com